StanCollender'sCapitalGainsandGames Washington, Wall Street and Everything in Between



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  • A New Twist on the Ownership Society   5 years 29 weeks ago

    MIT Phd and former Freddie economist onwhere we are now.

  • A New Twist on the Ownership Society   5 years 29 weeks ago

    Time to reboot the system

  • A Maverick Versus An Outsider For The White House   5 years 29 weeks ago

    When most Americans say our country is on the wrong track they don't mean: Politicians do not take positions contrary to their own party more often. They mean the country is on the wrong track.

    Was McCain a maverick when he originally opposed the Bush Tax cuts, or now that he thinks they should be permanent? Was he a maverick for supporting immigration reform or now because he says he would vote against his own bill? Was McCain a maverick when he opposed offshore drilling or now that he says we must drill drill drill? Was he a maverick for calling the religious right intolerant or now that he so successfully pandered to them? Was he a maverick when opposed earmarks or when he chose America's rooting tooting-ist earmark happy mayor to be his co-pilot and soul mate.

    So if you don't put your brand on it you can always claim anything unbranded belongs to you.

    I'm guessing you own an SUV, but now you are thinking really hard about a hybrid.

    So why are we on the wrong track again?

  • A New Twist on the Ownership Society   5 years 29 weeks ago

    Another simple change to enact is to change the bankruptcy/receivership clawback rules to include any management bonuses or stock grants/option sales for the previous 5 years.

    Today, if a company going into bankruptcy pays a bill to a creditor, that payment can be pulled back and distributed evenly to creditors. Payments to failed managers should be included in these clawbacks.

  • A Maverick Versus An Outsider For The White House   5 years 29 weeks ago

    CNN has Obama ahead in key states, and leading in electoral votes:

    http://www.cnn.com/2008/POLITICS/06/10/electoral.map/index.html

    The focus is on electoral votes; nationwide polls are relatively useless because popular vote totals aren't what wins the presidential election (as recent history shows)

    It's all about playing to get electoral votes. Minnesota and Iowa were added to the Obama column just last week:

    http://politicalticker.blogs.cnn.com/2008/09/03/electoral-map-alert-obam...

    Obama is gaining traction in my neck of the woods . . .

  • A Maverick Versus An Outsider For The White House   5 years 29 weeks ago

    McCain and the Republicans have so much going against them -- even Republicans are sick of Republicans this year -- that McCain should be 15 points down even in polls conducted by ouija board and dowsing stick.

    Dukakis was up by 17 points at this point. Obama has barely been able to draw above 45% even during his fleeting post-convention bump. Something is keeping his campaign from getting traction. That's just a fact.

  • A Maverick Versus An Outsider For The White House   5 years 29 weeks ago

    McCain has been very inept in managing his campaign.

    You have to be kidding. He just transformed the race by naming Super Sarah as his running mate.

  • This Week's "Fiscal Fitness"   5 years 29 weeks ago

    Thank you very much for this information.

  • A Maverick Versus An Outsider For The White House   5 years 29 weeks ago

    You don't have to win the popular vote in this election. I think that lesson was reinforced quite recently ;-)

    Look at electoral vote count. CNN has a chart on it.

    Also, remember that those are phone (land line) polls. Cell phone users aren't polled, and most people under 30 are now on cell-only . . . they are being undercounted and they are a huge base for Obama.

  • A New Twist on the Ownership Society   5 years 29 weeks ago

    The international US creditors own tons of those securities . . . so I guess they own us. Now let's see what happens -- further fall of the dollar, further inflation, job losses, more foreclosures, more bailouts -- a repeating cycle?

    I'm not an economist but this looks like a train wreck to me. The fact that this was necessary BEFORE the election speaks volumes about how bad it is. They were trying to wait to dump it on the next admin but couldn't.

  • A Maverick Versus An Outsider For The White House   5 years 29 weeks ago

    I thought McCain got his Maverick brand for standing up to the religious right....

    McCain showed he is willing to infuriate the entire Republican establishment by crossing party lines to work bipartisanly with Democrats on campaign finance reform, McCain-Feingold. Many on the right have not forgiven him to this day.

    I'd like to see an issue on which Obama is willing to similarly infuriate the Democratic establishment and left by working with Republicans. I'd enjoy seeing someone in one of the debates ask him to name that issue. It sure didn't seem to be "free trade" in light of the way Goolsbee got sent into exile for the rest of the primary season after talking to the Canadians. Could there be another one? No sign of it.

    McCain has been very inept in managing his campaign....

    That must be why today he's ahead in the Gallup tracking poll and tied in the Rasmussen poll, when by rights he should be 15 points down.

  • A New Twist on the Ownership Society   5 years 29 weeks ago

    If wealthy special interests can own it and gouge the public for obscene profits, it is something to privatize and they can own it.

    If it loses money big time, then the public has to bail it out and the public owns the loss.

    Privatization is one feature of a government of by and for special interests.

  • A Maverick Versus An Outsider For The White House   5 years 29 weeks ago

    The bailout of Freddie/Fannie that McCain supports will ensure that spending is not cut. More indication that he is not serious about cutting spending. The Bush tax cuts are not sustainable given the demands.

  • A Maverick Versus An Outsider For The White House   5 years 29 weeks ago

    "Senator John McCain is hardly the first maverick to make a rapid political ascent in America according to Safire."

    Rapid? He's been a member of Congress for 25 years.
    He's 72 years old. If that's rapid I'd like to see what slow is . . .

    Seems like Obama is the one who made a rapid political ascent.

  • A Maverick Versus An Outsider For The White House   5 years 29 weeks ago

    I thought McCain got his Maverick brand for standing up to the religious right. Now he is given into them and reigniting the culture wars.

    McCain has been very inept in managing his campaign. Would he do a lot better running our government? Plus McCain only really cares about the military and self-admittedly doesn't know much about the economy. I don't see how a McCain administration would be able to break with the Borrow and Spend policies of Bush? McCain talks about cutting spending, but he is not specific about what he would cut.

    Anyone who refuses to identify the cuts they would make to popular programs is NOT serious about cutting spending. Serious spending cuts will have to come from the military by freezing the military percent GDP the way Bush 41 and Clinton did it. There is no way a Commander in Chief McCain would cut the budget of his military. I don't see McCaim being an effective president.

  • A Tantalizing Preface   5 years 29 weeks ago

    Those books are also on my reading pile as well.

  • What Do You Call This?   5 years 29 weeks ago

    It should be the "b" word there on the bailout

  • What Do You Call This?   5 years 29 weeks ago

    Auto industry slump trickles through the entire midwest as there are many, many feeder industries.

    Housing/credit crisis is pretty much nationwide. Now the government is seizing Freddie and Fannie and placing them into conservatorship, which will cost taxpayers tens of millions (if not hundreds of millions) of dollars. They have to do this as these companies guarantee $5 trillion in securities and many of our foreign lender countries hold these securities. No other way out, but it spins us further into debt.

    Unemployment up, inflation up.

    Good luck to McCain.

  • What Do You Call This?   5 years 29 weeks ago

    Minnesota Mom

    A lot of the problems in the economy may be concentrated in certain geographic regions and industries, which may give McCain hope. Coupled with the electoral college system, this election is unpredictable and volatile.

  • What Do You Call This?   5 years 29 weeks ago

    I call this a huge defeat for the Republicans in November.

  • Your Weekly Dose Of "Fiscal Fitness"   5 years 30 weeks ago

    thanks post

  • Auto Bailout Picks Up Support   5 years 30 weeks ago

    First we have to recapitalize the banking system while still funding all the masters of the universe who milked it dry. Then we have to subsidize the idiots who paid too much for their houses. Now we have to pay off the moronic managerial class in Detroit who didn't understand that petroleum would become scarce.

    I don't think capitalism is working very well, and I'm not willing to pay to rescue it until we find a handful of grownups who can come up with a sensible roadmap away from our current predicament. Sadly, neither major party candidate yet passes the test. It goes without saying that Congress isn't close to figuring it out either.

  • In Praise of Mickey Edwards   5 years 30 weeks ago

    Thanks. Speaking as a person who used to vote Republican (before the extreme right-wing religious whackos took over the party), I'd love to see the party get back to basic conservative values!

    "Remember that we are a religious people but a secular nation."

    Yes!!!!! Republicans, please repeat this daily. My governor just came out in support of teaching creationism ("Intelligent Design") in public school science classes! I suppose he has to show his support for Palin (who also believes in this wingnut stuff), but geez.

    Could we get back to the real issues folks . . . like the housing crisis and failing economy? Rising unemployment? The budget deficit? War in Iraq? Bueller? Anybody?

    This campaign can't stay "on message" because they don't have one!

  • What the Palin Pick Says   5 years 30 weeks ago

    And I'd heard it from Republican party people (of the evangelist persuasion, no less!) . . . here are some saying it at higher levels

    http://politicalticker.blogs.cnn.com/2008/09/03/hot-mic-catches-gop-stra...

    And then check out the youtube of the "its over" moment

    http://www.youtube.com/watch?v=CrG8w4bb3kg

  • Does Anyone Believe In The Free Market Any More?   5 years 30 weeks ago

    i tend to get FIM Disease from time to time (foot-in-mouth disease), AND long-winded. you can have all the test in the world, they can be 100% validated, they can be proven, But, they are only as good as the ones giving them out i suppose. and in the past, this has proven to be a fruitless task with BSE and atypical TSEs et al at USDA, in fact, down right criminal. people may not want to talk politics, but politics are the very reason we sit where we sit, in terms of TSE of all species in the USA and abroad. i have spent 11 years daily watching it. and it aint getting no better. i will post this below, and if the moderator decides to remove it, so be it. but i am not making this stuff up. i will just post something from earlier today, and for those that care to read, i think you might find interest in how things work in the real world of TSEs here in the USA. CWD is one of those TSE in the USA. i cannot figure out the difference in testing for CWD between states, in comparing other TSEs, such as TSE in cattle, to TSE in sheep, and why the big difference in terms of of of ..... i think there is difference in how they allow testing in regards to ........... i don't even know what i am trying to say. the political issues are just different........... sometimes i do confuse myself........... but it's just handled differently it seems, and i could very well be wrong. i mean for instance, o.k. now bare with me. why quarantine a farm for 4 to 5 years due to an atypical TSE of foreign origin and due to potential serious animal and HUMAN health here in the USA there-from. then a few years later, discover the same damn TSE in the USA, home grown, and the same quarantine is not put in place. case in points, the mad sheep of mad river valley compared to those two atypical H-BSE cases in Texas and Alabama, and 6 cases to date of the atypical scrapie NOR-98 in six different states. why is the usda et al hiding behind some serum toxin act from decades ago, simply to NOT allow testing for BSE/TSE in the USA bovine? and why is the same serum-toxin act not applying to the CWD testing in the States, with deer and elk? or does it? these are not trick questions, i really want to know. you cannot ignore these other TSEs, and the politics that surround them. well, the moderators can ;-) and that's their choice, and i will abide by whatever move they take. i am not trying to get off topic, but, these issues must not be ignored. that must not happen with CWD in Michigan. and remember, in Terms of TSE i.e. prion disease, Michigan has CWD, Scrapie, and the atypical Nor-98 scrapie, Michigan also has cattle$ remember, the first recorded case of scrapie in the US occurred on a ranch in Michigan in 1947, the year of the first TME outbreak in Wisconsin. riddle me this batman, riddle me this ???

    see last link at bottome of page ; ''Bovine Spongiform Encephalopathy Mad Cow Disease typical and atypical strains, was there a cover-up ?''

    kind regards, terry

    xxxxxxxxxxx

    Prof Collinge old findings, a key issue, the media always seems to forget about, states that BSE propagates as either variant CJD-like or sporadic CJD-like prion strains. funny how our health officials, governments, and such seem to conveniently ignore these findings, let alone the atypical TSE in cattle and sheep that do NOT look like nvCJD in the lab, but some sub-types of the sporadic CJD. all this goes ignored for one reason, and one reason only $$$ it's why the UKBSEnvCJD only theory was put forth in the first place, and it's why it is still set in stone $$$ to much science has been forth to date that proves that theory wrong.

    PLEASE NOTE, the last two mad cows documented in the USA were H-type BSE, then the USDA quickly shut the testing down to a level of non-detection. it would be a miracle to find one case, from testing only 40,000 annually, from some 97,000,000 (that's 97 million head), and of that, some 37 million slaughtered, if i am not mistaken. and these cattle are cherry picked brains too, probably calves from USDA grain fed facilities, and don't think these corrupt people are not capable of doing it either. they are very capable. ...

    "REDACTED is alleged to have provided possibly inaccurate test results involving diseased sheep. However, because the results were determined to be inconclusive, no actual violation was actually committed.''

    http://foiamadsheepmadrivervalley.blogspot.com/2008/09/re-foia-of-declar...

    snip...

    Statement on Texas Cow With Central Nervous System Symptoms On Friday, April 30 th , the Food and Drug Administration learned that a cow with central nervous system symptoms had been killed and shipped to a processor for rendering into animal protein for use in animal feed.

    snip...

    I would note that the sample was taken in April, at which time the protocols allowed for a preservative to be used (protocols changed in June 2005). The sample was not submitted to us until last week, because the veterinarian set aside the sample after preserving it and simply forgot to send it in. On that point, I would like to emphasize that while that time lag is not optimal, it has no implications in terms of the risk to human health. The carcass of this animal was destroyed, therefore there is absolutely no risk to human or animal health from this animal.

    snip...

    Owner and Corporation Plead Guilty to Defrauding Bovine Spongiform Encephalopathy (BSE) Surveillance Program

    An Arizona meat processing company and its owner pled guilty in February 2007 to charges of theft of Government funds, mail fraud, and wire fraud. The owner and his company defrauded the BSE Surveillance Program when they falsified BSE Surveillance Data Collection Forms and then submitted payment requests to USDA for the services. In addition to the targeted sample population (those cattle that were more than 30 months old or had other risk factors for BSE), the owner submitted to USDA, or caused to be submitted, BSE obex (brain stem) samples from healthy USDA-inspected cattle. As a result, the owner fraudulently received approximately $390,000. Sentencing is scheduled for May 2007.

    snip...

    4 USDA OIG SEMIANNUAL REPORT TO CONGRESS FY 2007 1st Half

    http://www.usda.gov/oig/webdocs/sarc070619.pdf

    full text ;

    http://foiamadsheepmadrivervalley.blogspot.com/2008/09/re-foia-of-declar...

    USDA: In 9,200 cases only one type of test could be used

    WASHINGTON (AP)--The U.S. Department of Agriculture acknowledged Aug. 17 that its testing options for bovine spongiform encephalopathy were limited in 9,200 cases despite its effort to expand surveillance throughout the U.S. herd.

    In those cases, only one type of test was used--one that failed to detect the disease in an infected Texas cow.

    The department posted the information on its website because of an inquiry from The Associated Press.

    Conducted over the past 14 months, the tests have not been included in the department's running tally of BSE tests since last summer. That total reached 439,126 on Aug. 17.

    "There's no secret program," the department's chief veterinarian, John Clifford, said in an interview. "There has been no hiding, I can assure you of that."

    Officials intended to report the tests later in an annual report, Clifford said.

    These 9,200 cases were different because brain tissue samples were preserved with formalin, which makes them suitable for only one type of test--immunohistochemistry, or IHC.

    In the Texas case, officials had declared the cow free of disease in November after an IHC test came back negative. The department's inspector general ordered an additional kind of test, which confirmed the animal was infected.

    Veterinarians in remote locations have used the preservative on tissue to keep it from degrading on its way to the department's laboratory in Ames, Iowa. Officials this year asked veterinarians to stop using preservative and send fresh or chilled samples within 48 hours.

    The department recently investigated a possible case of BSE that turned up in a preserved sample. Further testing ruled out the disease two weeks ago.

    Scientists used two additional tests--rapid screening and Western blot--to help detect BSE in the country's second confirmed case, in a Texas cow in June. They used IHC and Western blot to confirm the first case, in a Washington state cow in December 2003.

    "The IHC test is still an excellent test," Clifford said. "These are not simple tests, either."

    Clifford pointed out that scientists reran the IHC several times and got conflicting results. That happened, too, with the Western blot test. Both tests are accepted by international animal health officials.

    Date: 8/25/05

    http://www.hpj.com/archives/2005/aug05/aug29/BSEtestoptionswerelimited.cfm

    ""These 9,200 cases were different because brain tissue samples were preserved with formalin, which makes them suitable for only one type of test--immunohistochemistry, or IHC."

    THIS WAS DONE FOR A REASON!

    THE IHC test has been proven to be the LEAST LIKELY to detect BSE/TSE in the bovine, and these were probably from the most high risk cattle pool, the ones the USDA et al, SHOULD have been testing. ...TSS

    USDA 2003

    We have to be careful that we don't get so set in the way we do things that we forget to look for different emerging variations of disease. We've gotten away from collecting the whole brain in our systems. We're using the brain stem and we're looking in only one area. In Norway, they were doing a project and looking at cases of Scrapie, and they found this where they did not find lesions or PRP in the area of the obex. They found it in the cerebellum and the cerebrum. It's a good lesson for us. Ames had to go back and change the procedure for looking at Scrapie samples. In the USDA, we had routinely looked at all the sections of the brain, and then we got away from it. They've recently gone back. Dr. Keller: Tissues are routinely tested, based on which tissue provides an 'official' test result as recognized by APHIS.

    Dr. Detwiler: That's on the slaughter. But on the clinical cases, aren't they still asking for the brain? But even on the slaughter, they're looking only at the brainstem. We may be missing certain things if we confine ourselves to one area.

    snip.............

    Dr. Detwiler: It seems a good idea, but I'm not aware of it. Another important thing to get across to the public is that the negatives do not guarantee absence of infectivity. The animal could be early in the disease and the incubation period. Even sample collection is so important. If you're not collecting the right area of the brain in sheep, or if collecting lymphoreticular tissue, and you don't get a good biopsy, you could miss the area with the PRP in it and come up with a negative test. There's a new, unusual form of Scrapie that's been detected in Norway. We have to be careful that we don't get so set in the way we do things that we forget to look for different emerging variations of disease. We've gotten away from collecting the whole brain in our systems. We're using the brain stem and we're looking in only one area. In Norway, they were doing a project and looking at cases of Scrapie, and they found this where they did not find lesions or PRP in the area of the obex. They found it in the cerebellum and the cerebrum. It's a good lesson for us. Ames had to go back and change the procedure for looking at Scrapie samples. In the USDA, we had routinely looked at all the sections of the brain, and then we got away from it. They've recently gone back.

    Dr. Keller: Tissues are routinely tested, based on which tissue provides an 'official' test result as recognized by APHIS .

    Dr. Detwiler: That's on the slaughter. But on the clinical cases, aren't they still asking for the brain? But even on the slaughter, they're looking only at the brainstem. We may be missing certain things if we confine ourselves to one area.

    snip...

    FULL TEXT;

    Completely Edited Version PRION ROUNDTABLE

    Accomplished this day, Wednesday, December 11, 2003, Denver, Colorado

    2005

    =============================

    CDC DR. PAUL BROWN TSE EXPERT COMMENTS 2006

    The U.S. Department of Agriculture was quick to assure the public earlier this week that the third case of mad cow disease did not pose a risk to them, but what federal officials have not acknowledged is that this latest case indicates the deadly disease has been circulating in U.S. herds for at least a decade.

    The second case, which was detected last year in a Texas cow and which USDA officials were reluctant to verify, was approximately 12 years old.

    These two cases (the latest was detected in an Alabama cow) present a picture of the disease having been here for 10 years or so, since it is thought that cows usually contract the disease from contaminated feed they consume as calves. The concern is that humans can contract a fatal, incurable, brain-wasting illness from consuming beef products contaminated with the mad cow pathogen.

    "The fact the Texas cow showed up fairly clearly implied the existence of other undetected cases," Dr. Paul Brown, former medical director of the National Institutes of Health's Laboratory for Central Nervous System Studies and an expert on mad cow-like diseases, told United Press International. "The question was, 'How many?' and we still can't answer that."

    Brown, who is preparing a scientific paper based on the latest two mad cow cases to estimate the maximum number of infected cows that occurred in the United States, said he has "absolutely no confidence in USDA tests before one year ago" because of the agency's reluctance to retest the Texas cow that initially tested positive.

    USDA officials finally retested the cow and confirmed it was infected seven months later, but only at the insistence of the agency's inspector general.

    "Everything they did on the Texas cow makes everything USDA did before 2005 suspect," Brown said. ...snip...end

    http://www.upi.com/ConsumerHealthDaily/view.php?StoryID=20060315-055557-...

    CDC - Bovine Spongiform Encephalopathy and Variant Creutzfeldt ... Dr. Paul Brown is Senior Research Scientist in the Laboratory of Central Nervous System ... Address for correspondence: Paul Brown, Building 36, Room 4A-05, ...

    http://www.cdc.gov/ncidod/eid/vol7no1/brown.htm

    PLEASE NOTE ;

    179 Page 10 of 17

    BSE cattle may need to be reexamined.

    T. Kitamoto (Ed.) PRIONS Food and Drug Safety

    ================

    ALSO from the International Symposium of Prion Diseases held in Sendai, October 31, to November 2, 2004; Bovine spongiform encephalopathy (BSE) in Japan

    snip...

    "Furthermore, current studies into transmission of cases of BSE that are atypical or that develop in young cattle are expected to amplify the BSE prion" NO. Date conf. Farm Birth place and Date Age at diagnosis 8. 2003.10.6. Fukushima Tochigi 2001.10.13. 23 9. 2003.11.4. Hiroshima Hyogo 2002.1.13. 21

    Test results # 8b, 9c cows Elisa Positive, WB Positive, IHC negative, histopathology negative b = atypical BSE case c = case of BSE in a young animal b,c, No PrPSc on IHC, and no spongiform change on histology International Symposium of Prion Diseases

    held in Sendai, October 31, to November 2, 2004. Tetsuyuki Kitamoto Professor and Chairman Department of Prion Research Tohoku University School of Medicine 2-1 SeiryoAoba-ku, Sendai 980-8575, JAPAN TEL +81-22-717-8147 FAX +81-22-717-8148 e-mail; kitamoto@mail.tains.tohoku.ac.jp Symposium Secretariat Kyomi Sasaki TEL +81-22-717-8233 FAX +81-22-717-7656 e-mail: kvomi-sasaki@mail.tains.tohoku.ac.ip ================================= 9/13/2005 -------------------------------------------------------------------------------- Page 11 of 17 From: TSS () Subject: Atypical Proteinase K-Resistant Prion Protein (PrPres) observed in an Apparently Healthy 23-Month-Old Holstein Steer Date: August 26, 2005 at 10:24 am PST Atypical Proteinase K-Resistant Prion Protein (PrPres) observed in an Apparently Healthy 23-Month-Old Holstein Steer Jpn. J. Infect. Dis., 56, 221-222, 2003 Laboratory and Epidemiology Communications Atypical Proteinase K-Resistant Prion Protein (PrPres) Observed in an Apparently Healthy 23-Month-Old Holstein Steer Yoshio Yamakawa*, KenÕichi Hagiwara, Kyoko Nohtomi, Yuko Nakamura, Masahiro Nishizima ,Yoshimi Higuchi1, Yuko Sato1, Tetsutaro Sata1 and the Expert Committee for BSE Diagnosis, Ministry of Health, Labour and Welfare of Japan2 Department of Biochemistry & Cell Biology and 1Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640 and 2Miistry of Health, Labour and Welfare, Tokyo 100-8916 Communicated by Tetsutaro Sata (Accepted December 2, 2003) *Corresponding author: Mailing address: Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 1628640, Japan. Tel: +81-3-5285-1111, Fax: +81-3-5285-1157, E-mail: yamakawa@nih.go.jp

    Since October 18, 2001, 'bovine spongiform encephalopathy (BSE) examination for all cattle slaughtered at abattoirs in the country' has been mandated in Japan by the Ministry of Health, Labour and Welfare (MHLW). 'Plateria' ELISA-kit (Bio-Rad Laboratories, Hercules, Calif., USA) is routinely used at abattoirs for detecting proteinase K (PK)-resistant prion protein (PrPSc) in the obex region. Samples positive according to the ELISA screening are further subjected to Western blot (WB) and histologic and immunohistochemical examination (IHC) at the National Institute of Infectious Diseases (NIID) or Obihiro University. If PrPSc is detected either by WB or by IHC, the cattle are diagnosed as BSE. The diagnosis is approved by the Expert Committee for BSE Diagnosis, MHLW. From October 18, 2001 to September 30, 2003, approximately 2.5 million cattle were screened at abattoirs. A hundred and ten specimens positive according to ELISA were subjected to WB/IHC. Seven showed positive by both WB and IHC, all exhibiting the typical electrophoretic profile of a high content of the di-glycosylated molecular form of PrPSc (1-3) and the distinctive granular deposition of PrPSc in neuronal cells and neuropil of the dorsal nucleus of vagus. An ELISA-positive specimen from a 23 month-old Holstein steer slaughtered on September 29, 2003, in Ibaraki Prefecture (Ibaraki case) was sent to the NIID for confirmation. The animal was reportedly healthy before slaughter. The OD titer in ELISA was slightly higher than the 'cut-off' level given by the manufacturer. The histology showed no spongiform changes and IHC revealed no signal of PrPSc accumulation typical for BSE. However, WB analysis of the homogenate that was prepared from the obex region and used for ELISA revealed a small amount of PrPSc with an electrophoretic profile different from that of typical BSE-associated PrPSc (1-3). The characteristics were (i) low content of the di-glycosylated molecular form of PrPSc, (ii) a faster migration of the non-glycosylated form of PrPSc on SDS-PAGE, and (iii) less resistance against PK digestion as compared with an authentic PrPSc specimen derived from an 83-month-old Holstein (Wakayama case) (Fig. 1). Table 1 summarizes the relative amounts of three distinctive glycoforms (di-, mono, non-glycosylated) of PrPSc calculated by densitometric analysis of the blot shown in Fig. 1. As 2.5 mg wet weight obex-equivalent homogenate of the Ibaraki case (Fig. 1, lane 4) gave slightly stronger band intensities of PrPSc than an 8 mg wet weight obex-equivqlent homogenate of a typical BSE-affected Wakayama case (Fig. 1, lane 2), the amount of PrPSc accumulated in the Ibaraki case was calculated to be 1/500 - 1/1000 of the Wakayama case. In the Ibaraki case, the PrPSc bands were not detectable in the homogenates of the proximal surrounding region of the obex. These findings were consistent with the low OD value in ELISA, i.e., 0.2 -0.3 for the Ibaraki case versus over 3.0 for the Wakayama case. The DNA sequence of the PrP coding region of the Ibaraki case was the same as that appearing in the database (GenBank accession number: AJ298878). More recently, we encountered another case that resembled the Ibaraki case. It was a 21-monthold Holstein steer from Hiroshima Prefecture. WB showed typical BSE-specific PrPSc deposition though IHC did not detect positive signals of PrPSc (data not shown). Though the clinical onset of BSE is usually at around 5 years of age or later, a 20-month-old case showing the clinical signs has been reported (4). Variant forms of BSE similar to our cases, i.e., with atypical histopathological and/or biochemical phenotype, have been recently reported in Italy (5) and in France (6). Such variant BSE was not associated with mutations in the prion protein (PrP) coding region as in our case (5,6). The Ministry of Agriculture, Forestry and Fisheries of Japan (MAFF) announced a ban of feeding ruminants with meat bone meal (MBM) on September 18, 2001, and a complete ban was made on October 15 of the same year. According to the recent MAFF report, the p evious seven cases of BSE in Japan were cattle born in 1995 - 1996 and possibly fed with cross-contaminated feed. However, the two cattle in this report were born after the complete ban. Whether contaminated MBM was implicated in the present cases remains to be investigated.

    REFERENCES Collinge, J., Sidle, K. C. L., Meads, J., Ironside, J. and Hill, A. F. (1996): Molecular analysis of prion strain variation and the aetiology of 'new variant' CJD. Nature, 383, 685690. Bruce, M. E., Will, R. G., Ironside, J. W., McConnell, I., Drummond, D., Suttie, A., McCardle, L., Chree, A., Hope, J., Birkett, C., Cousens, S., Fraser, H. and Bostock, C. J. (1997): Transmissions to mice indicate that 'new variant' CJD is caused by the BSE agent. Nature, 389, 498-501. Hill, A. F., Desbruslais, M., Joiner, S., Sidle, K. C. L., Gowland, I. and Collinge, J. (1997): The same prion strain causes vCJD and BSE. Nature, 389, 448-450. Matravers, W., Bridgeman, J. and Smith, M.-F. (ed.)(2000): The BSE Inquiry. p. 37. vol. 16. The Stationery Office Ltd., Norwich, UK. Casalone, C., Zanusso, G., Acutis, P. L., Crescio, M. I., Corona, C., Ferrari, S., Capobianco, R., Tagliavini, F., Monaco, S. and Caramelli, M. (2003): Identification of a novel molecular and neuropathological BSE phenotype in Italy. International Conference on Prion Disease: from basic research to intervention concepts. Gasreig, Munhen, October 8-10. Bicaba, A. G., Laplanche, J. L., Ryder, S. and Baron, T. (2003): A molecular variant of bovine spongiform encephalopatie. International Conference on Prion Disease: from basic research to intervention concepts. Gasreig, Munhen, October 8-10. Asante, E. A., Linehan, J. M., Desbruslais, M., Joiner, S., Gowland, I., Wood, A. L., Welch, J., Hill, A. F., Lloyd, S. E., Wadsworth, J. D. F. and Collinge, J. (2002). BSE prions propagate as either variant CJD-like or sporadic CJD-like prion strains in transgenic mice expressing human prion protein. EMBO J., 21, 6358-6366. 9/13/2005 Page 12 of 17 SEE SLIDES IN PDF FILE; http://www.nih.go.jp/JJID/56/221.pdf

    http://www.fsis.usda.gov/OPPDE/Comments/03-025IFA/03-025IFA-2.pdf

    THE SEVEN SCIENTIST REPORT ***

    http://www.fda.gov/ohrms/dockets/dockets/02n0273/02n-0273-EC244-Attach-1...

    ***

    WELL, someone did call me from Bio-Rad about this, however it was not Susan Berg. but i had to just about take a blood oath not to reveal there name. IN fact they did not want me to even mention this, but i feel it is much much to important. I have omitted any I.D. of this person, but thought I must document this ;

    Bio-Rad, TSS phone conversation 12/28/04

    Finally spoke with ;

    Bio-Rad Laboratories 2000 Alfred Nobel Drive Hercules, CA 94547 Ph: 510-741-6720 Fax: 510-741-5630 Email: XXXXXXXXXXXXXXXXXX

    at approx. 14:00 hours 12/28/04, I had a very pleasant phone conversation with XXXX XXXXX about the USDA and the inconclusive BSE testing problems they seem to keep having. X was very very cautious as to speak directly about USDA and it's policy of not using WB. X was very concerned as a Bio-Rad official of retaliation of some sort. X would only speak of what other countries do, and that i should take that as an answer. I told X I understood that it was a very loaded question and X agreed several times over and even said a political one.

    my question;

    Does Bio-Rad believe USDA's final determination of False positive, without WB, and considering the new atypical TSEs not showing positive with -IHC and -HP ???

    ask if i was a reporter. i said no, i was with CJD Watch and that i had lost my mother to hvCJD. X did not want any of this recorded or repeated.

    again, very nervous, will not answer directly about USDA for fear of retaliation, but again said X tell me what other countries are doing and finding, and that i should take it from there. "very difficult to answer"

    "very political"

    "very loaded question"

    outside USA and Canada, they use many different confirmatory tech. in house WB, SAF, along with IHC, HP, several times etc. you should see at several talks meetings (TSE) of late Paris Dec 2, that IHC- DOES NOT MEAN IT IS NEGATIVE. again, look what the rest of the world is doing. said something about Dr. Houston stating; any screening assay, always a chance for human error. but with so many errors (i am assuming X meant inconclusive), why are there no investigations, just false positives? said something about ''just look at the sheep that tested IHC- but were positive''. ...

    TSS

    -------- Original Message -------- Subject: Your questions Date: Mon, 27 Dec 2004 15:58:11 -0800 From: To: flounder@wt.net

    Hi Terry:

    ............................................snip Let me know your phone number so I can talk to you about the Bio-Rad BSE test. Thank you

    Regards

    Bio-Rad Laboratories 2000 Alfred Nobel Drive Hercules, CA 94547 Ph: 510-741-6720 Fax: 510-741-5630 Email: =================================

    END...TSS

    ######### https://listserv.kaliv.uni-karlsruhe.de/warc/bse-l.html ##########

    http://madcowtesting.blogspot.com/

    Executive Summary

    In June 2005, an inconclusive bovine spongiform encephalopathy (BSE) sample from November 2004, that had originally been classified as negative on the immunohistochemistry test, was confirmed positive on SAF immunoblot (Western blot). The U.S. Department of Agriculture (USDA) identified the herd of origin for the index cow in Texas; that identification was confirmed by DNA analysis. USDA, in close cooperation with the Texas Animal Health Commission (TAHC), established an incident command post (ICP) and began response activities according to USDA’s BSE Response Plan of September 2004. Response personnel removed at-risk cattle and cattle of interest (COI) from the index herd, euthanized them, and tested them for BSE; all were negative. USDA and the State extensively traced all at-risk cattle and COI that left the index herd. The majority of these animals entered rendering and/or slaughter channels well before the investigation began. USDA’s response to the Texas finding was thorough and effective.

    http://www.aphis.usda.gov/lpa/issues/bse/epi-updates/bse_final_epidemiol...

    i hope i have not lost you. i know how some don't like to get political, but cwd, mad cow disease (all strains), TME, Scrapie, and cjd i.e. human and animal TSE, that's all they are are political. bush has failed us terribly, clinton before him failed us terribly, and whomever gets in office next will do the same damn thing, in terms of human and animal TSE. it was said long ago ;

    In Confidence - Perceptions of unconventional slow virus diseases of animals in the USA - APRIL-MAY 1989 - G A H Wells

    3. Prof. A Robertson gave a brief account of BSE. The US approach was to accord it a very low profile indeed. Dr. A Thiermann showed the picture in the ''Independent'' with cattle being incinerated and thought this was a fanatical incident to be avoided in the US at all costs. BSE was not reported in the USA.

    http://www.bseinquiry.gov.uk/files/mb/m11b/tab01.pdf

    PLEASE NOTE, ''FANATICAL INCIDENT TO BE AVOIDED IN THE US AT ALL COSTS.''

    and they meant it. ...TSS

    The EMBO Journal Vol. 21 No. 23 pp. 6358±6366, 2002

    BSE prions propagate as either variant CJD-like or sporadic CJD-like prion strains in transgenic mice expressing human prion protein

    Emmanuel A.Asante, Jacqueline M.Linehan, Melanie Desbruslais, Susan Joiner, Ian Gowland, Andrew L.Wood, Julie Welch, Andrew F.Hill, Sarah E.Lloyd, Jonathan D.F.Wadsworth and John Collinge1 MRC Prion Unit and Department of Neurodegenerative Disease, Institute of Neurology, University College, Queen Square, London WC1N 3BG, UK 1Corresponding author e-mail: j.collinge@prion.ucl.ac.uk Variant Creutzfeldt±Jakob disease (vCJD) has been recognized to date only in individuals homozygous for methionine at PRNP codon 129. Here we show that transgenic mice expressing human PrP methionine 129, inoculated with either bovine spongiform encephalopathy (BSE) or variant CJD prions, may develop the neuropathological and molecular phenotype of vCJD, consistent with these diseases being caused by the same prion strain. Surprisingly, however, BSE transmission to these transgenic mice, in addition to producing a vCJD-like phenotype, can also result in a distinct molecular phenotype that is indistinguishable from that of sporadic CJD with PrPSc type 2. These data suggest that more than one BSEderived prion strain might infect humans; it is therefore possible that some patients with a phenotype consistent with sporadic CJD may have a disease arising from BSE exposure. ...snip...end...TSS

    Molecular Features of the Protease-resistant Prion Protein (PrPres) in H-type BSE

    Biacabe, A-G1; Jacobs, JG2; Gavier-Widén, D3; Vulin, J1; Langeveld, JPM2; Baron, TGM1 1AFSSA, France; 2CIDC-Lelystad, Netherlands; 3SVA, Sweden

    Western blot analyses of PrPres accumulating in the brain of BSE-infected cattle have demonstrated 3 different molecular phenotypes regarding to the apparent molecular masses and glycoform ratios of PrPres bands. We initially described isolates (H-type BSE) essentially characterized by higher PrPres molecular mass and decreased levels of the diglycosylated PrPres band, in contrast to the classical type of BSE. This type is also distinct from another BSE phenotype named L-type BSE, or also BASE (for Bovine Amyloid Spongiform Encephalopathy), mainly characterized by a low representation of the diglycosylated PrPres band as well as a lower PrPres molecular mass. Retrospective molecular studies in France of all available BSE cases older than 8 years old and of part of the other cases identified since the beginning of the exhaustive surveillance of the disease in 20001 allowed to identify 7 H-type BSE cases, among 594 BSE cases that could be classified as classical, L- or H-type BSE. By Western blot analysis of H-type PrPres, we described a remarkable specific feature with antibodies raised against the C-terminal region of PrP that demonstrated the existence of a more C-terminal cleaved form of PrPres (named PrPres#2 ), in addition to the usual PrPres form (PrPres #1). In the unglycosylated form, PrPres #2 migrates at about 14 kDa, compared to 20 kDa for PrPres #1. The proportion of the PrPres#2 in cattle seems to by higher compared to the PrPres#1. Furthermore another PK–resistant fragment at about 7 kDa was detected by some more N-terminal antibodies and presumed to be the result of cleavages of both N- and C-terminal parts of PrP. These singular features were maintained after transmission of the disease to C57Bl/6 mice. The identification of these two additional PrPres fragments (PrPres #2 and 7kDa band) reminds features reported respectively in sporadic Creutzfeldt-Jakob disease and in Gerstmann-Sträussler-Scheinker (GSS) syndrome in humans. ...end

    USA MAD COW STRAIN MORE VIRULENT TO HUMANS THAN UK STRAIN

    18 January 2007 - Draft minutes of the SEAC 95 meeting (426 KB) held on 7 December 2006 are now available.

    snip...

    64. A member noted that at the recent Neuroprion meeting, a study was presented showing that in transgenic mice BSE passaged in sheep may be more virulent and infectious to a wider range of species than bovine derived BSE.

    Other work presented suggested that BSE and bovine amyloidotic spongiform encephalopathy (BASE) MAY BE RELATED. *** A mutation had been identified in the prion protein gene in an AMERICAN BASE CASE THAT WAS SIMILAR IN NATURE TO A MUTATION FOUND IN CASES OF SPORADIC CJD.

    snip...

    http://www.seac.gov.uk/minutes/95.pdf

    AND WHAT ABOUT THOSE atypical NOR-98 scrapie cases in the USA, now documented at 6 cases in the past two years, and the risk thereof to humans as sporadic CJD ???

    P03.141

    Aspects of the Cerebellar Neuropathology in Nor98

    Gavier-Widén, D1; Benestad, SL2; Ottander, L1; Westergren, E1 1National Veterinary Insitute, Sweden; 2National Veterinary Institute,

    Norway Nor98 is a prion disease of old sheep and goats. This atypical form of scrapie was first described in Norway in 1998. Several features of Nor98 were shown to be different from classical scrapie including the distribution of disease associated prion protein (PrPd) accumulation in the brain. The cerebellum is generally the most affected brain area in Nor98. The study here presented aimed at adding information on the neuropathology in the cerebellum of Nor98 naturally affected sheep of various genotypes in Sweden and Norway. A panel of histochemical and immunohistochemical (IHC) stainings such as IHC for PrPd, synaptophysin, glial fibrillary acidic protein, amyloid, and cell markers for phagocytic cells were conducted. The type of histological lesions and tissue reactions were evaluated. The types of PrPd deposition were characterized. The cerebellar cortex was regularly affected, even though there was a variation in the severity of the lesions from case to case. Neuropil vacuolation was more marked in the molecular layer, but affected also the granular cell layer. There was a loss of granule cells. Punctate deposition of PrPd was characteristic. It was morphologically and in distribution identical with that of synaptophysin, suggesting that PrPd accumulates in the synaptic structures. PrPd was also observed in the granule cell layer and in the white matter. The pathology features of Nor98 in the cerebellum of the affected sheep showed similarities with those of sporadic Creutzfeldt-Jakob disease in humans.

    INFECTED AND SOURCE FLOCKS

    There were 20 scrapie infected and source flocks with open statuses (Figure 3) as of April, 30, 2008. Twenty eight new infected and source flocks have been designated in FY 2008 (Figure 4); three source flocks were reported in April. ...snip

    POSITIVE SCRAPIE CASES

    As of April 30, 2008, 122 new scrapie cases have been confirmed and reported by the National Veterinary Services Laboratories (NVSL) in FY 2008 (Figure 6). Of these, 103 were field cases and 19* were Regulatory Scrapie Slaughter Surveillance (RSSS) cases (collected in FY 2008 and reported by May 20, 2008). Positive cases reported for April 2008 are depicted in Figure 7. Eighteen cases of scrapie in goats have been confirmed by NVSL since implementation of the regulatory changes in FY 2002 (Figure 8). The most recent positive goat case was confirmed in February 2008 and originated from the same herd in Michigan as the other FY 2008 goat cases. ...snip

    CAPRINE SCRAPIE PREVALENCE STUDY (CSPS)

    snip...

    However, four positive goats have been identified this fiscal year through field investigations. One was a clinical suspect submitted for testing and the other three originated from the birth herd of the clinical case.

    ANIMALS SAMPLED FOR SCRAPIE TESTING

    As of April 30, 2008, 26,703 animals have been sampled for scrapie testing: 23,378 RSSS, 1,517 goats for the CSPS study, 1,466 regulatory field cases, 270 regulatory third eyelid biopsies, and 72 regulatory rectal biopsies (chart 8).

    TESTING OF LYMPHOID TISSUE OBTAINED BY RECTAL BIOPSY WAS APPROVED BY USDA AS AN OFFICIAL LIVE-ANIMAL TEST ON JANUARY 11, 2008. ...

    PLEASE NOTE, (FIGURE 6), Scrapie Confirmed Cases in FY 2008 MAP, PA 3, 1**, Two cases-state of ID UNKNOWN, 1 case Nor98-like**

    http://www.aphis.usda.gov/animal_health/animal_diseases/scrapie/download...

    NOT to forget the 5 cases of the NOR-98 atypical scrapie documented in the USA in 2007, in five different states. WHICH pathologically looks like some sub-types of sporadic CJD, of which Stanely Prusiner warns of a public health risk ;

    ***The pathology features of Nor98 in the cerebellum of the affected sheep showed similarities with those of sporadic Creutzfeldt-Jakob disease in humans.

    http://www.prion2007.com/pdf/Prion%20Book%20of%20Abstracts.pdf

    Here we report that both Nor98 and discordant cases, including three sheep homozygous for the resistant PrPARR allele (A136R154R171), efficiently transmitted the disease to transgenic mice expressing ovine PrP, and that they shared unique biological and biochemical features upon propagation in mice. These observations support the view that a truly infectious TSE agent, unrecognized until recently, infects sheep and goat flocks and may have important implications in terms of scrapie control and public health.

    Edited by Stanley B. Prusiner, University of California, San Francisco, CA, and approved September 12, 2005 (received for review March 21, 2005)

    http://www.pnas.org/cgi/content/abstract/0502296102v1

    Tuesday, June 3, 2008 SCRAPIE USA UPDATE JUNE 2008 NOR-98 REPORTED PA

    http://nor-98.blogspot.com/2008/06/scrapie-usa-update-june-2008-nor-98.html

    NOR-98 ATYPICAL SCRAPIE 5 cases documented in USA in 5 different states USA 007

    http://nor-98.blogspot.com/2008/04/seac-spongiform-encephalopathy-adviso...

    http://nor-98.blogspot.com/

    Nonambulatory Cattle as a Potential Source of TSE

    In this study, Wisconsin was the only State in which mink producers were reported to receive nonambulatory cows directly from dairies. However, given the small number of surveyed herds this finding is likely a result of the sampling design. Because mink producers pay a premium for nonambulatory cows, it appears reasonable that the practice of feeding nonambulatory cows to mink could occur wherever both large numbers of dairy cows and mink are found. As many as 2,157(3) nonambulatory cows per million milk cows, or a total of 9,482 nonambulatory cows, could have been fed to mink in the 7 surveyed States in 1992. Based on the sample response, only half of those cows would have had an identifiable reason for being nonambulatory. This equates to an estimated 4,741 nonambulatory cows that were, hypothetically, a potential source of TSE in the surveyed States.

    (3)This estimate does not account for any nonambulatory cows received from slaughter plants.

    Page 23

    The five reported outbreaks of TME in the U.S. reveal no discernable trend. Assuming an average of 2,000 mink farms in the U.S. during the last 50 years, one outbreak of TME has occurred per 20,000 mink farm-years. Extrapolating from the data gathered in this study, 66,374 nonambulatory cows have been fed to mink in the 7 surveyed States since the last reported outbreak of TME in 1985. Of those, 33,187 would have had no identifiable reason for being nonambulatory and were hypothetically a potential source of TSE. Given the severity of signs and number of mink affected by TME it is unlikely that outbreaks have gone unreported. If any form Of a TSE (infectious, spontaneous, or other) occurs in U.S. cattle that is transmissible to mink in the form of TME, then it must be exceedingly rare or the conditions for its transmission must be highly specific and unusual. Nonetheless, studies are underway at the State and Federal levels to further characterize the disposition of nonambulatory cows and usage on mink farms.

    snip...

    An estimated 4,741 nonambulatory cows hypothetically considered to be potential sources of TSE may have been fed to mink in the 7 surveyed States in 1992. This equates to 33,187 such cows fed to mink since the last reported outbreak of TME in mink. Given this large number of nonambulatory cows fed to mink, the historic and current mink population, and the infrequent occurrence of TME, if TSE exists in cattle in the U.S. it must be very rare or transmissible to mink only under very unusual conditions.

    http://downercattle.blogspot.com/2008/08/quantitative-assessment-of-poss...

    To be published in the Proceedings of the Fourth International Scientific Congress in Fur Animal Production. Toronto, Canada, August 21-28, 1988

    Evidence That Transmissible Mink Encephalopathy Results from Feeding Infected Cattle

    R.F. Marsh* and G.R. Hartsough

    •Department of Veterinary Science, University of Wisconsin-Madison, Madison, Wisconsin 53706; and ^Emba/Creat Lakes Ranch Service, Thiensville, Wisconsin 53092

    ABSTRACT

    Epidemiologic investigation of a new incidence of transmissible mink encephalopathy (TME) in Stetsonville, Wisconsin suggests that the disease may have resulted from feeding infected cattle to mink. This observation is supported by the transmission of a TME-like disease to experimentally inoculated cattle, and by the recent report of a new bovine spongiform encephalopathy in England.

    snip...

    OBSERVATIONS AND RESULTS

    A New Incidence of TME. In April of 1985, a mink rancher in Stetsonville, Wisconsin reported that many of his mink were “acting funny”, and some had died. At this time, we visited the farm and found that approximately 10% of all adult mink were showing typical signs of TME: insidious onset characterized by subtle behavioral changes, loss of normal habits of cleanliness, deposition of droppings throughout the pen rather than in a single area, hyperexcitability, difficulty in chewing and swallowing, and tails arched over their _backs like squirrels. These signs were followed by progressive deterioration of neurologic function beginning with locomoior incoordination, long periods of somnolence in which the affected mink would stand motionless with its head in the corner of the cage, complete debilitation, and death.

    Over the next 8-10 weeks, approximately 40% of all the adult mink on the farm died from TME. Since previous incidences of TME were associated with common or shared feeding practices, we obtained a careful history of feed ingredients used over the past 12-18 months. ***The rancher was a “dead stock” feeder using mostly (>95%) downer or dead dairy cattle and a few horses. Sheep had never been fed.***

    snip...end

    http://www.bseinquiry.gov.uk/files/mb/m09/tab05.pdf

    Epidemiology Epidemiologic studies suggest that animals contract the disease by external exposure to the infectious agent, such as by eating contaminated feed. No evidence suggests that the TME agent spreads by contact between unrelated mink or from mother to nursing young. The disease has been identified in both genders and all color phases in animals greater than 1 year old. The first documented TME outbreak in the United States occurred in 1947 on one ranch in Wisconsin and then on a ranch in Minnesota that had received mink from the Wisconsin ranch. In 1961, TME outbreaks occurred on five ranches in Wisconsin. In Factsheet Veterinary Services February 2002 APHIS 1963, outbreaks occurred in Idaho, Minnesota, and Wisconsin. Epidemiologic data from the Minnesota and Wisconsin outbreaks trace the cases in those States to one common purchased food source.

    snip...

    The 1985 Stetsonville Outbreak The most recent TME outbreak occurred on one mink ranch in Stetsonville, WI, in 1985. In the herd of 7,300 adult mink, 60 percent of the animals died. Clinical signs included tail arching, incoordination, and hyperexcitability. At the most advanced stages of the disease, the animals were in trancelike states and eventually died. The outbreak lasted 5 months. Microscopic examination of sections of the brain confirmed the spongelike changes characteristic of TME. Diagnostic tests identified the prion protein. The following year, mink born during the outbreak showed no signs of TME. The late Richard Marsh, a veterinary virologist at the University of Wisconsin who studied the transmission of TME and other TSE’s, investigated this outbreak. Marsh learned that the mink were fed a diet composed of fresh meat products from “downer cattle” and commercial sources of fish, poultry, and cereal. Downer cattle are nonambulatory and cannot rise because they are affected with a condition such as a metabolic disease, broken limbs, or a central nervous system disorder. Marsh theorized that the meat from these downer cattle introduced a TSE agent to the mink in which TME resulted. Although Marsh’s hypothesis is based on speculation and anecdotal evidence, in 1993 APHIS adjusted its national BSE surveillance program to include testing downer cattle for evidence of a TSE. The brains of more than 20,141 cattle have been examined at APHIS’ National Veterinary Services Laboratories and other State diagnostic laboratories. Not a single tissue sample has revealed evidence of BSE or another TSE in cattle.

    http://www.aphis.usda.gov/publications/animal_health/content/printable_v...

    AND as everyone knows, the rest is history, those dead-stock downers, the most high risk cattle, were NOT tested, and in FACT, was a major source of YOUR CHILDRENS SCHOOL LUNCH PROGRAM, all across the Nation. sorry, these are the most high risk cattle for TSE aka mad cow disease, and i am a bit touchy about this topic. ...sorry. ...terry

    DOWNER COW SCHOOL LUNCH PROGRAM

    http://downercattle.blogspot.com/

    IS THERE A SCRAPIE-LIKE DISEASE IN CATTLE ?

    In April of 1985, a mink rancher in Wisconsin reported a debilitating neurologic disease in his herd which we diagnosed as TME by histopathologic findings confirmed by experimental transmission to mink and squirrel monkeys. The rancher was a ''dead stock'' feeder using mostly (>95%) downer or dead dairy cattle and a few horses. She had never been fed.

    We believe that these findings may indicate the presence of a previously unrecognized scrapie-like disease in cattle and wish to alert dairy practitioners to this possibility.

    snip...

    PROCEEDINGS OF THE SEVENTH ANNUAL WESTERN CONFERENCE FOR FOOD ANIMAL VETERINARY MEDICINE, University of Arizona, March 17-19, 1986

    http://www.bseinquiry.gov.uk/files/mb/m09a/tab01.pdf

    http://www.bseinquiry.gov.uk/files/mb/m09/tab05.pdf

    NOW, back to those mad mink i.e. TME. let me throw a curve ball here ;

    Phenotypic Similarity of Transmissible Mink Encephalopathy in Cattle and L-type Bovine Spongiform Encephalopathy in a Mouse Model Thierry Baron,* Anna Bencsik,* Anne-Gaëlle Biacabe,* Eric Morignat,* and Richard A. Bessen† Emerging Infectious

    Transmissible mink encepholapathy (TME) is a foodborne transmissible spongiform encephalopathy (TSE) of ranch-raised mink; infection with a ruminant TSE has been proposed as the cause, but the precise origin of TME is unknown. To compare the phenotypes of each TSE, bovine- passaged TME isolate and 3 distinct natural bovine spongiform encephalopathy (BSE) agents (typical BSE, Htype BSE, and L-type BSE) were inoculated into an ovine transgenic mouse line (TgOvPrP4). Transgenic mice were susceptible to infection with bovine-passaged TME, typical BSE, and L-type BSE but not to H-type BSE. Based on survival periods, brain lesions profi les, disease-associated prion protein brain distribution, and biochemical properties of protease-resistant prion protein, typical BSE had a distint phenotype in ovine transgenic mice compared to L-type BSE and bovine TME. The similar phenotypic properties of L-type BSE and bovine TME in TgOvPrP4 mice suggest that L-type BSE is a much more likely candidate for the origin of TME than is typical BSE. Transmissible mink encephalopathy (TME) is a rare prion disease in ranch-raised mink (Mustela vison) in North America and Europe (1–4). Six outbreaks have been reported from 1947 through 1985 in North America, and several have been linked to contaminated commercial feed (1). Although contamination of feed with scrapie-infected sheep parts has been proposed as the cause of TME, the origin of the disease remains elusive. The idea that scrapie in sheep may be a source of TME infection is supported by fi ndings that scrapie-infected mink have a similar distribution of vacuolar pathologic features in the brain and the same clinical signs as mink with natural and experimental TME (5). However, mink are not susceptible to scrapie infection following oral exposure for up to 4 years postinoculation, which suggests that either the scrapie agent may not be the source of natural TME infection or that only specifi c strains of the scrapie agent are able to induce TME (6,7). Epidemiologic investigations in the Stetsonville, Wisconsin, outbreak of TME in 1985 suggested a possible cattle origin, since mink were primarily fed downer or dead dairy cattle but not sheep products (8). Experimental transmission of Stetsonville TME into cattle resulted in transmissible spongiform encephalopathy (TSE) disease with an incubation period of 18.5 months. Back passage of bovine TME into mink resulted in incubation periods of 4 and 7 months after oral or intracerebral inoculation, respectively, which was similar to that found following inoculation of Stetsonville TME into mink by these same routes (8). These fi ndings indicated that cattle are susceptible to TME, and that bovine-passaged TME did not result in a reduced pathogenicity for mink. These studies raised the question as to whether an unknown TSE in cattle was the source of TME infection in the Stetsonville outbreak. Several additional TME outbreaks in the United States have been associated with mink diet that contained downer or dead cattle (9). ...

    snip...full text ;

    http://www.cdc.gov/EID/content/13/12/pdfs/1887.pdf

    http://transmissible-mink-encephalopathy.blogspot.com/

    CWD, is but a small piece, of a very big puzzle. and one of the main reasons this puzzle has not been solved, is the secrecy, cover-ups, and such. you must not ignore these facts. i did not want to believe this either. for years i was naive, but the facts speak for themselves.

    it goes way back ;

    Wednesday, August 20, 2008

    Bovine Spongiform Encephalopathy Mad Cow Disease typical and atypical strains, was there a cover-up ?

    August 20, 2008

    http://bse-atypical.blogspot.com/2008/08/bovine-spongiform-encephalopath...

    Wednesday, September 03, 2008

    Accelerated High Fidelity Prion Amplification Within and Across Prion Species Barriers

    http://chronic-wasting-disease.blogspot.com/2008/09/accelerated-high-fid...

    to be continued. ...TSS



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